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. 2017 Mar 22;75(3):ftx035. doi: 10.1093/femspd/ftx035

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Published by Oxford University Press on behalf of FEMS 2017. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 3. — Comparative proteomics of chxR mutants. (A) A Venn diagram showing proteins downregulated in chxR mutant strains compared to WT organisms. Compared to WT, proteins CT005, CT214, CT565, CT694 and CT695 were downregulated in all three chxR mutants. (B) Western blot validation of proteome results. Lysates of WT and the three chxR null mutants were electrophoresed on SDS-PAGE gels and immunoblotted using ChxR, CT005 and chlamydial HSP60 antibodies. All three chxR mutants did not react with αChxR mAb or anti-CT005.