Table 6.
Comparison of genotyping cost (USD) considering advancement of all QTL classes versus advancement of only higher performing F3 generation QTL classes
| Background | Generation | Number of QTL classes | Population size | Cost (USD) | Total genotyping cost (USD) | Savings (USD) | |||
|---|---|---|---|---|---|---|---|---|---|
| Based on all classes | Based on selected classes | Based on all classes | Based on selected classes | Based on all classes | Based on selected classes | ||||
| Swarna-Sub1 | F3 | 15 | 754 | 754 | 5655 | 5655 | 21,420 | 8978 | 12,443 |
| F4 | 15 | 754 | 106 | 5655 | 795 | ||||
| F5 | 10 | 432 | 106 | 3240 | 795 | ||||
| F6 | 10 | 432 | 106 | 3240 | 795 | ||||
| F7 | 6 | 432 | 108 | 3240 | 810 | ||||
| F8 | 5 | 52 | 17 | 390 | 127.50 | ||||
| IR64-Sub1 | F3 | 20 | 467 | 467 | 7005 | 7005 | 12,105 | 8385 | 3720 |
| F4 | 19 | 194 | 46 | 2910 | 690 | ||||
| F5 | 19 | 64 | 18 | 960 | 270 | ||||
| F6 | 13 | 64 | 18 | 960 | 270 | ||||
| F7 | 7 | 18 | 10 | 270 | 150 | ||||
| Samba Mahsuri | BC1F3 | 3 | 42 | 42 | 210 | 210 | 21,760 | 6980 | 14,780 |
| BC1F4 | 3 | 3000 | 640 | 15,000 | 3200 | ||||
| BC1F5 | 3 | 1200 | 640 | 6000 | 3200 | ||||
| BC1F6 | 3 | 70 | 44 | 350 | 220 | ||||
| BC1F7 | 2 | 20 | 15 | 100 | 75 | ||||
| BC1F8 | 2 | 20 | 15 | 100 | 75 | ||||
| TDK1-Sub1 | BC2F3 | 14 | 843 | 843 | 6323 | 6323 | 9225 | 6954 | 2272 |
| BC2F4 | 7 | 231 | 43 | 1733 | 323 | ||||
| BC2F5 | 7 | 48 | 14 | 360 | 105 | ||||
| BC2F6 | 7 | 48 | 14 | 360 | 105 | ||||
| BC2F7 | 5 | 60 | 13 | 450 | 98 | ||||
| MR219 | BC1F3 | 7 | 214 | 214 | 1605 | 1605 | 11,955 | 5730 | 6225 |
| BC1F4 | 7 | 620 | 240 | 4650 | 1800 | ||||
| BC1F5 | 7 | 620 | 240 | 4650 | 1800 | ||||
| BC1F6 | 7 | 70 | 35 | 525 | 262.50 | ||||
| BC1F7 | 7 | 70 | 35 | 525 | 262.50 | ||||
The genotyping cost was calculated considering five markers per QTL (one peak/near the peak, two right-hand-side flanking markers, and two left-hand-side flanking markers) and USD 0.50 per data point