Fig. 3.

HDL and 4F promote HUVECs directional migration. (A) HUVECs were plated on FN-coated glass bottom culture dishes and labeled with 1 μM, 5-chloromethylfluorescein diacetate. After cells were incubated with HDL, Cl/NO₂-HDL at 100 µg/mL apoA-1 and/or 4F at 50 µg/mL concentration for 6 h, time-lapse microscopy was performed on a fluorescent microscope (10× objective) with Nikon BioStation IM (10× objective). The red arrow follows the labeled cell across various time points. (B) Cell speed was measured in 20 random fields with ImageJ using the Manual Tracking plug-in. Cells migration into the gaps were quantified. Videos were shown in supplemental movie S1. These experiments have three independent biological replicates and HUVECs were isolated from three fresh cords and passages 2–5 was used.