Fig. 4.

Effects of 4F on the formation of lamellipodia in HUVECs and HAECs. HAECs(A) and HUVECs(B) were treated with native HDL, Cl/NO₂-HDL at 100 µg/mL apoA-1 and/or 4F at 50 µg/mL concentration for 15 min, and confocal images of lamellipodia in HUVECs are shown. HUVECs were stained with rhodamine phalloidin (red) and DAPI (blue). HAEC (C) and HUVEC (D) migrated cells were counted in 15 random high-power (200×) fields under varied conditions. The experiments were performed as six independent biological replicates and every replicate have three technical replicates.