Synopsis of time course and experimental design of the study. Experiment 1: Study of the effect of RH on ischemic acidosis in ITD rats. Study groups were Naïve, ITD, and ITD + RH. Experiment 2: Study of the effect of inhibition of acidosis on ischemic injury in RH-exposed ITD rats. Study groups were ITD + RH, and ITD + RH + THAM. Experiment 3: Study of the effect of GLUT-inhibition on ischemic injury in RH-exposed ITD rats. Study groups were ITD + RH + Vehicle, and ITD + RH + GLUT inhibitor. ITD: insulin-treated diabetic, RH: recurrent hypoglycemia, THAM: Tris-(hydroxymethyl)-aminomethane.