Skip to main content
. 2017 Dec 8;15:159–169. doi: 10.1016/j.redox.2017.11.025

Fig. 2.

Fig. 2

Different miRNA and mRNA expression profiles and qRT-PCR detectionin vivoandin vitroand luciferase activity assay. (A) Heat map with intuitive reflection of the features of expression profiles changes in the control group and Se-deficient group. (B) Myocardial tissue miRNA levels of miR-200a-5p in the control group and Se-deficient group. Bars represent the mean ± SD of 30 individuals. Bars represent the mean ± SD of triplicate cell cultures. *p < 0.05 by Student's t-test. (C) To investigate the most appropriate transfection concentration in cardiomyocytes model with transfecting different concentrations of miR-200a-5p-mimic, miR-200a-5p-inhibitor, mimic negative control and inhibitor negative control for 24 h. miRNA levels of miR-200a-5p was determined by RT-PCR. The optimum concentration of miR-200a-5p-mimic and miR-200a-5p-inhibitor used in this paper were always 100 nM and 50 nM, respectively. Bars that do not share the same letters are significantly different (p < 0.05) from each other. The data were expressed as the mean ± SD of triplicate cell cultures. (D) The mRNA levels of RNF11 in the cardiomyocytes transfected with mimic, inhibitor and control cardiomyocytes. Bars that do not share the same letters are significantly different (p < 0.05) from each other. The data were expressed as the mean ± SD of triplicate cell cultures. (E) The mRNA and protein levels of RNF11 in the control myocardial tissue and Se-deficient myocardial tissue. Bars represent the mean ± SD of 30 individuals. Bars represent the mean ± SD of triplicate cell cultures. *p < 0.05 by Student's t-test. (F–G) Correlation between RNF11 and miR-200a-5p in luciferase reporter gene assay results. pMIR-RNF11-WT plasmids was mutated in the miRNA target sites, and designated as pMIR-RNF11-Mut. miR-200a-5p-mimic inhibits RNF11-WT expression but not mutant RNF11-MUT expression. Bars that do not share the same letters are significantly different (p < 0.05) from each other. The data were expressed as the mean ± SD of triplicate cell cultures.