Different mRNA expression of TNF-α-related genes in vivo and in vitro. (A) The mRNA expression of TAB, TAK1, RIP2, TNF-α, NF-κb and RNF11 in the control myocardial tissue and Se-deficient myocardial tissue. Bars represent the mean ± SD of 30 individuals. *p < 0.05 by Student's t-test. (B) The mRNA levels of TAB, TAK1, RIP2, TNF-α, NF-κb and RNF11 in cardiomyocytes transfected with mimic and inhibitor and control cardiomyocytes. Bars represent the mean ± SD of triplicate cell cultures. Bars that do not share the same letters are significantly different (p < 0.05) from each other.