Fig. 4.

Src and Erk, the downstream signals of CR3, mediate paraquat and maneb-induced NOX2 activation. (A) The levels of phosphorylated and total Src and Erk were determined in paraquat and maneb-treated BV2 microglia by Western blot using specific antibodies and the representative blots were shown. (B) The activation of Src and Erk was quantified by analysis of the blot density. (C) The effects of saracatinib (Src inhibitor) and U0126 (Erk inhibitor) on P+M-induced production of intracellular superoxide were measured using DHE and the density of red fluorescence was quantified. (D) The effects of saracatinib and U0126 on P+M-induced production of extracellular superoxide was measured. (E) The effects of saracatinib and U0126 on P+M-induced membrane translocation of p47^phox was detected using Western blot and the density of blots was quantified. Results were expressed as a percentage of controls from three experiments performed in duplicate. *p < 0.05, **p < 0.01. Bar = 30 µm.