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. 2017 Dec 15;15:207–215. doi: 10.1016/j.redox.2017.12.003

Fig. S2.

Fig. S2

The effect of calcium phosphate (CaP) and cystine crystals on MnSOD gene expression and protein levels in THP-1 cells. (A and B) Real-time PCR was performed to determine MnSOD and GAPDH expression in THP-1 cells treated with CaP or cystine crystals. MnSOD was normalized to GAPDH (housekeeping gene). Relative fold change was calculated using the DDCT method relative to control samples. (C and D) Representative western blot of MnSOD (25 kDa) and GAPDH (37 kDa) protein levels in THP-1 cells treated with CaP or cystine crystals. Results are means ± SEM; n = 3–5 individual experiments. *p < 0.05 compared to untreated monocytes.