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Disruption of lacs in H. irregularis. (A) Strategy for targeted disruption of lacs. (B) Verification of the correct mutant by PCR. (C) HPLC analysis of the products of the wild type and mutant on the oatmeal agar plates at 210 nm. (i) wild type; (ii) mutant; (iii) standard of 1; (iv) standard of 2.
