Figure 4.

Heterogeneity of IL-8 induction among multiple individual H. pylori colonies isolated from each monkey. (A) Multiple isolates from within most individual monkeys showed similar induction of IL-8, though in some cases (e.g. monkeys 1 and 5) there was considerable variability. (B) Biological replicates of strains recovered from monkey 5 showed that the isolate with the highest IL-8 (open circle) was consistently higher than all others. The cagPAI sequence of this isolate was later compared to one of the isolates that induced low IL-8 (open square). IL-8 values are normalized to the results for H. pylori strain J166.