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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: ChemMedChem. 2017 Dec 4;12(23):2006–2013. doi: 10.1002/cmdc.201700626

Figure 3.

Figure 3

Non-radioactive cellular cytotoxicity assay using the compound 31. (A,B) Expansion of NK cells. Peripheral blood mononuclear cells from two healthy donors were each incubated with anti-CD3 monoclonal antibody-conjugated beads and the CD3+ T cells removed. The CD3− cells were incubated with IL-2 and the expanded NK cells were analyzed for CD56 expression by flow cytometry. (C,D) Determination of the specific lysis of K562 cells by NK cell lines. K562 cells labeled with 31 were incubated with the NK cell line from panel A for panel C or NK cells from panel B for panel D at effector to target ratios of 0, 0.625, 1.25, 2.5, 5, 10, 20 and 40. The specific lysis (%) was calculated as described in the Materials and Methods. Mean ± SD is shown for triplicate values.