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Purification of CT1087NHis6 is enabled by the addition of TX-100 to Ni-NTA elution buffer. (A) Coomassie stained SDS-PAGE gel. (B) Immunoblot with α-His4 antibody. Lane labels: L = MW ladder, CSP = cholate solubilized membrane protein, FT = Ni-NTA agarose column flow through during binding, Elution = fractions following elution with buffer containing TX-100, Biobeads = the indicated amounts of protein (μg) from elution fraction 11 after removal of TX-100.
