Figure 1.
Deletion of the putative periplasmic of PilJ (pilJΔ74-273) reduces twitching motility and intracellular cAMP levels. (A) Diameter of twitching motility zones for the indicated strains. Nine colonies were assayed to determine the average and standard error of the mean. Asterisks (***) indicate values significantly different from wild type (P < 0.001) as determined by ANOVA followed by TukeyHSD. Non-significant differences are indicated by n.s. (B) SDS-PAGE showing the surface levels of PilA obtained from the indicated strains. (C) Whole cell levels of PilA as determined by western blotting for the indicated strains. (D) Intracellular cAMP levels of the indicated strains expressed as Miller Units. Three colonies were assayed in triplicate to determine the average and the standard error of the mean. Asterisks (***) indicate significantly different values (P < 0.001) as determined by ANOVA followed by TukeyHSD. Non-significant differences are indicated by n.s.