Figure 3.
Providing cAMP through a cyaB point mutant restores wild-type levels of twitching motility in pilJΔ74-273. (A) Zones of twitching motility for the indicated strains. Nine colonies were assayed to determine the average and standard error of the mean. (B) SDS-PAGE showing the surface levels of PilA obtained from the indicated strains. (C) Whole cell levels of PilA as determined by western blotting for the indicated strains. (D) Intracellular cAMP levels of the indicated strains expressed as Miller Units. Three colonies were assayed in triplicate to determine the average and the standard error of the mean. The strains contained either pJN105 (V) or pJN105-cyaBR412H (cyaBR412H) as indicated. In panels A and D, asterisks (***, P < 0.001) indicate significantly different values as determined by ANOVA followed by TukeyHSD. Non-significant differences are indicated by n.s.