Figure 5.

PilJ is not absolutely required for directional twitching to PE provided sufficient cAMP is present. (A) Representative images of directional twitching results. The PE or stearic acid was deposited on the plate to the right of where the P. aeruginosa culture was placed. Scale bar = 1 mm (0.8× magnification) (B) Directional twitching ratios were calculated by dividing the length of the leading edge by the length of the lagging edge. Three independent colonies were analyzed in triplicate. A ratio greater than 2 indicates directional twitching. Significantly different values were determined by ANOVA followed by TukeyHSD (*, P < 0.05).