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Analysis of PDIP1 mRNA expression by multiple-tissue Northern blotting. A preblotted membrane with mRNAs from nine different human tissues was probed with a 1.6-kb PDIP1 cDNA, randomly labeled with [α-32P]dCTP as described in Materials and Methods. After stripping, the membrane was reprobed with a 2.0-kb cDNA of a β-actin gene fragment. PDIP1 mRNA levels were normalized to β-actin mRNA and quantitated by using National Institutes of Health IMAGE version 1.62 software.
