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PDIP1 simultaneously binds to PCNA and p50. PCNA and p50 were mixed with GST-PDIP1 or GST and processed as described in Materials and Methods. Lane 1, PCNA control; lane 2, p50 control; lane 3, GST, p50, and PCNA; lane 4, GST-PDIP1, p50, and PCNA. PCNA was detected with PC10 as primary antibody, and p50 was detected with rabbit anti-p50 as primary antibody.
