FIGURE 6.

Effects of DM on HFD-induced obesity in C57BL/6 mice. DM (250 or 500 mg/kg) or orlistat (50 mg/kg) were administered by oral gavage for 8 weeks while the mice were fed the HFD. (A) Experimental outline. (B) Body weight was measured 3 times per week. The group that received HFD alone () showed steady body weight gain, while the DM-( or ) and orlistat-treated () groups showed significantly attenuated body weight gain. (C) Adipose tissue weight of subcutaneous, epididymal, and mesentery fat. (D) Amount of leptin in plasma measured by ELISA. (E) Liver weight. (F) Plasma levels of GOT and GPT measured using a chemical analyzer. (G) H&E-stained images of liver and subcutaneous adipose tissue samples from the normal diet (control), vehicle-treated, DM-treated, and orlistat-treated groups. The results are expressed as the mean ± SD for each group (n = 8). ^#p < 0.05 compared with the control group; ^∗p < 0.05 compared with the HFD-treated vehicle group.