Fig. 1.

Expression of ERRβ in normal vs breast cancer tumor samples, cell lines and its pathological significance. a Immunohistochemical staining of tissue microarray slides using ERRβ antibody in both normal (n = 4) and breast carcinoma tissues (n = 19). Increased expression of ERRβ in normal tissues compared with breast carcinoma. b Graphical representation of IHC composite score of each tissue microarray sample. Composite score was calculated for each sample using both intensity score and percentage of cells positive for ERRβ staining (composite score < 3 low categorized; 3–5 moderately categorized; ≥ 6 highly categorized). Graph was plotted using composite score and p-values were calculated using 2-group t-test (p < 0.05 considered as significant). c Western blots revealing high expression of ERRβ in normal breast cell line (MCF10A), than breast cancer cell lines (MCF7, T47D, and MDA-MB-231). Densitometry analyses of ERRβ expression in normal and breast cancer cell lines, One-way ANOVA test was performed to acquire statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). d Kaplan-Meier survival curve of Chanrion et al. (Dataset: GSE9893) correlated higher expression of ERRβ with favorable survival (p = 0.027382)