Fig. 3.

Expression of ERRβ is ERα dependent. Efficient knockdown of ERα showing significant decrease in the expression of ERRβ in MCF7 cells. a, b Quantitative Real-time PCR (qRT-PCR) and Reverse transcription polymerase chain reaction (RT-PCR) results showing decreased expression of ERRβ in ERα depleted MCF7 cells. Housekeeping gene GAPDH treated as control and ΔCt, ΔΔCt, 2^-ΔΔCt values were calculated and graph was plotted using 2^-ΔΔCt values. Fold change ≥ 2 was considered as significant. p-values were calculated using 2-group t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). c Western blot revealing the depleted expression of ERRβ in ERα Knockdown MCF7 (ERα KD) cells