Table 1.

Comparison of different staining methods

staining method	Giemsa conventional	May-Gruenwald-Giemsa modified
used solution	Giemsa’s azur-eosin-methylene blue solution	May-Gruenwald’s eosin-methylene blue solution modified, Giemsa’s azur-eosin-methylene blue solution
staining cell nucleus	bluish	reddish - violet
staining cytoplasm	reddish	from bluish to reddish - violet
staining mucus	light purple	bluish
staining microbial colonies	Pink to reddish	reddish to purple
staining artifacts	blue to purple	blue to pink
staining background	light pink	clear