Table 1.
Identification of differentially expressed proteins in Sf9 cells treated with the azadirachtin by LTQ-MS/MS
| Spot no | Protein name | Fold change a) | NCBI accession number | DP b) | AAC c) (%) |
MW (Da) |
PI | Peptides identified |
|---|---|---|---|---|---|---|---|---|
| D1 | DnaJ homolog subfamily A member 1 | 1.61 ± 0.13 | gi|14053203 | 2 | 2.00 | 45134.93 | 6.38 | SGNDLILR |
| D2 | Ribosomal protein SA | 2.85 ± 0.18 | gi|54609281 | 3 | 7.84 | 33408.77 | 4.87 | FAAHTGATPIAGR |
| D3 | Actin | 8.97 ± 0.21 | gi|46371991 | 6 | 13.70 | 40603.37 | 5.46 | GYSFTTTAER |
| D4 | Actin | 3.21 ± 0.11 | gi|46371991 | 7 | 16.99 | 40603.37 | 5.46 | GYSFTTTAER |
| D5 | Actin | 1.92 ± 0.08 | gi|46371991 | 7 | 16.99 | 40603.37 | 5.46 | GYSFTTTAER |
| D6 | Beta-tubulin | 10.43 ± 0.24 | gi|74275413 | 3 | 10.14 | 33323.12 | 5.9 | EVDEQMLNIQNK |
| D7 | Proteasome zeta subunit | 6.26 ± 0.23 | gi|114050993 | 7 | 36.63 | 26874.64 | 4.98 | LFQVEYAIEAIK |
| D8 | P27BBP/eIF6-like | 3.17 ± 0.14 | gi|82880642 | 4 | 14.69 | 26304.74 | 4.63 | VQFENNNEVGVFSK |
| D9 | Translationally-controlled tumor protein homolog | 2.38 ± 0.16 | gi|74837218 | 10 | 41.86 | 19938.81 | 4.67 | LVETYAFGDKK |
| D11 | Proteasome subunit alpha type 6-A | 1.73 ± 0.07 | gi|114052160 | 3 | 13.41 | 27143 | 6.44 | GTDAAVVAAQR |
| U1 | Ribosomal protein L9 | 5.06 ± 0.19 | gi|112983495 | 6 | 25.79 | 21377.03 | 9.94 | MAPGVTVVNSPK |
| U2 | Abnormal wing disc-like | 2.80 ± 0.22 | gi|153791847 | 5 | 27.92 | 17312.92 | 6.75 | NIIHGSDSVESAK |
a) Fold change: D1-D11: the ratio of protein intensity of CK versus AZA; U1-U2: the ratio of protein intensity of AZA versus CK.
b) Distinct peptides matched. c) Amino acids coverage.