Table 3.

Characteristics of primary antibodies and staining details

Antibody	Antigen	Species	Origin details	Dilution	Incubation time	Antigen retrieval	Detection method
pTau, clone AT8	Tau phosphorylated at Ser202 and Thr205	Mouse IgG1	ThermoFisher, Pittsburgh, USA	1:10000	32 min at 36 °C	Heat-induced (pH 8.5) for 24 min	Optiview
Amyloid-beta, clone IC-16	N-terminus of amyloid-beta (aa 1-16)	Mouse IgG2a	Dr. Carsten Korth, University of Dusseldorf, Germany	1:25	32 min at 36 °C	Heat-induced (pH 8.5) for 24 min	Optiview
GFAP, clone EP672Y	Glial fibrillary acidic protein	Mouse	Roche, Basel, Switzerland	1: 2	8 min at 37 °C	Heat-induced (pH 8.5) for 32 min	Optiview
Iba1	C-terminus of Iba1	Rabbit	Wako Pure Chemical Industries, Osaka, Japan	1:4000	32 min at 36 °C	Heat-induced (pH 8.5) for 16 min	Optiview
CD68, clone KP1	CD68	Mouse IgG1	Dako, Glostrup, Denmark	1:1200	Overnight at 4°C	Heat-induced (pH 6.0) by autoclave	EnVision
HLA-DP/DQ/DR, clone CR3/43	Alpha and beta-chains of all products of the DP, DQ, and DR subregions	Mouse IgG1	Dako	1:800	Overnight at 4°C	Heat-induced (pH 6.0) by autoclave	EnVision
C1q	C1q	Rabbit	Dako	1:25600	Overnight at 4°C	Heat-induced (pH 6.0) by autoclave	EnVision
C3d	C3d	Rabbit	Dako	1:3200	Overnight at 4°C	Heat-induced (pH 6.0) by autoclave	EnVision
C4b	C4b	Rabbit	Abcam, Cambridge, United Kingdom	1:1600	Overnight at 4°C	Heat-induced (pH 6.0) by autoclave	EnVision
C5b-9, clone WU13-15	Neoepitope on C9 in the membrane attack complex	Mouse	Hycult Biotech, Plymouth meeting, USA	1:400	Overnight at 4°C	Heat-induced (pH 6.0) by autoclave	EnVision