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. 2018 May 29;18:32. doi: 10.1186/s12896-018-0442-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Frozen DEAB exposure in developing zebrafish embryos. Embryos exposed to DEAB at 5 μM that have been stored for 4 months at -20 °C in either DMSO (b), G3TFSA (c), or G4TFSA (d) display the reported characteristics of loss of RA (compared to control (DMSO exposed only (a): lack of pectoral fin induction (arrowhead), shortening of the posterior head (asterisk), malformation of the otic vesical (arrow) and pericardial edema