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Tagmentation on surface. a oligonucleotides are attached to a ~ 10 μm thick polyacrylamide gel. The 5’ end of the oligos have an acrydite modification for attachment to the acrylamide matrix. b The Tn5 transposases are assembled on the dsDNA oligos on the polyacrylamide gel surface. c genomic DNA is linked to the surface through tagmentation via incubation at 55 °C for 20 min with TAPs buffer. d, e side view and vertical view of the linked DNA on surface
