Fig. 3.

t-AUCB prevented upregulation of miR-133 and restored the expression of KCNQ1 and KCNH2 mRNA in ischemic myocardium. a Ischemic upregulated miR-133 expression in MI hearts, while t-AUCB suppressed miR-133 expression in a dose-dependent manner. miR-133 level were quantificated by real-time PCR with RNA samples isolated from mice hearts 24 h after MI. b The upregulation of miR-133 was exacerbated by agomir in MI hearts, but alleviated by t-AUCB. c Ischemic downregulated KCNQ1 and KCNH2 mRNA expression in MI hearts, while t-AUCB restored KCNQ1 and KCNH2 mRNA expression in a dose-dependent manner. d Levels of both KCNQ1 and KCNH2 mRNA expression were reduced in MI and the reduction was exacerbated by agomir-133, but alleviated by t-AUCB. Data were expressed as mean ± SEM; *P<0.05 vs. sham group; #P<0.05 vs. MI group; &P<0.05 vs agomir-133 + MI group, n = 5