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. 2018 May 29;17:129. doi: 10.1186/s12944-018-0780-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — SRF signaling pathway participated in regulation of miR-133 by sEHi. a Ischemic upregulated SRF mRNA expression in MI hearts, while t-AUCB suppressed SRF mRNA expression in a dose-dependent manner. SRF mRNA level were quantificated by real-time PCR with RNA samples isolated from mice hearts 24 h after MI. b Ischemic downregulated SRF protein expression in MI hearts, while t-AUCB restored SRF protein expression in a dose-dependent manner. Measurements were made 24 h after MI. Top, examples of western blot bands; Bottom, relative expression level of SRF protein. Quantitation as mean ± SEM. *P<0.05 vs. sham group; #P<0.05 vs. MI group; n = 5