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. 2018 May 30;19:191. doi: 10.1186/s12859-018-2191-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Read counts and dispersion distribution greatly influence the estimated sample size and power. a The read counts and dispersion distribution for all genes from TCGA Rectum adenocarcinoma (READ) dataset. The red lines indicate read counts equal to one and 10. And the green line indicates the 95% quantile of all gene dispersions. b The estimated sample size required to achieve 0.8 power in different combinations of read counts and dispersions