Figure 4.

Upregulation of lipogenic enzyme SCD1 in CPT2-depleted HepG2 cells. (A) qRT-PCR analysis for mRNA expression of lipogenic enzyme genes ACLY, ACC1, FASN, and SCD1 in HepG2 cells as indicated. Data are presented as the mean ± SD from three independent experiments. (B) Western blot analysis for protein levels of SCD1 in the indicated cells. (C) Schematic model depicting the activation of lipogenesis by CPT2 downregulation which in turn promotes proliferation, metastasis, and chemoresistance in HCC. *P<0.05; **P<0.01.

Abbreviations: CPT2, carnitine palmitoyltransferase-2; HCC, hepatocellular carcinoma; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.