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Primer sequences for qPCR
| Gene | Primer name | Sequence (5′–3′) | Product size (bp) |
|---|---|---|---|
| CPT2 | F | CTGTAGCACTGCCGCATTCA | 213 |
| R | AGAGCAAACAAGTGTCGGTCAA | ||
| CPT1A | F | GACGTGGGAAAAATAAGCAGTC | 202 |
| R | ATCTTCCCGTTTTTGAAGACAA | ||
| ACLY | F | CAGCAGGACAGCATCTTTTTC | 97 |
| R | TGGACTTGGGACTGAATCTTG | ||
| ACC1 | F | AGTGAGGATGGCAGCTCTGGA | 132 |
| R | TGAGATGTGGGCAGCATGAAC | ||
| FASN | F | TGAACGCCGGCACCAATA | 97 |
| R | GACTGGTACAACGAGCGGATGA | ||
| SCD1 | F | GCAGGACGATATCTCTAGCT | 93 |
| R | GTCTCCAACTTATCTCCTCCATTC | ||
| Actin | F | CCACGAAACTACCTTCAACTCC | 174 |
| R | GGAGCAATGATCTTGATCTTCA |
