Fig. 4.

Whole genome sequencing revealed a point mutation (1 base pair deletion) in exon 20 of the canine DMD gene. a Screen shot of NCBI Genome Workbench revealing 22 reads with the point mutation (nucleotide A; black rectangle). b Screen shot of Leiden DMD database with the deleted nucleotide highlighted in blue (red arrow). A stop codon (TGA) present six nucleotides downstream in exon 20 (red line). c Sanger sequencing screen shot of the mutated area (black arrow) with the reverse strain ACT stop codon six nucleotides downstream (black line). d Sanger sequencing screen shot of a normal dog in the same area. Black arrow points at the normal (non-mutated) sequence