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Detection limits of one-step RT-PCR and RtF-RT-LAMP for the NK94P6 strain
| TCID50 | 2.8 × 105 | 2.8 × 104 | 2.8 × 103 | 2.8 × 102 | 2.8 × 101 | 2.8 × 100 | 2.8 × 10− 1 |
|---|---|---|---|---|---|---|---|
| One-step RT-PCR |
+ | + | + | – | – | – | |
| RtF-RT-LAMP (amplification time mm:ss) |
+ (9:30) |
+ (11:45) |
+ (12:30) |
+ (15:45) |
+ (34:45) |
– | – |
+ Positive in duplicate
- Negative in duplicate
From 2.8 × 105 to 2.8 × 10− 1: tenfold serial dilution of 2.8 × 106 TCID50 PEDV NK94P6 strain
