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Detection limits of one-step RT-PCR and RtF-RT-LAMP for the Fukuoka-1 Tr(−) strain
| TCID50 | 2 × 103 | 2 × 102 | 2 × 101 | 2 × 100 | 2 × 10− 1 | 2 × 10− 2 | 2 × 10− 3 |
|---|---|---|---|---|---|---|---|
| One-step RT-PCR | + | + | – | – | – | – | – |
| RtF-RT-LAMP (amplification time mm:ss) |
+ 16:00 |
+ 18:15 |
+ 23:45 |
+ 35:45 |
± | – | – |
+ Positive in duplicate
- Negative in duplicate
± One positive and one negative in duplicate
From 2 × 103 to 2 × 10− 3: tenfold serial dilution of 2 × 104 TCID50 PEDV Fukuoka-1 Tr(−) strain
