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Chemical shifts of the Uxs1 assay product in D2O at 25°C (in ppm), referenced to internal acetone at δ2.218 relative to 3-(trimethylsilyl)-1-propanesulfonic acid
| H1 | H2 | H3 | H4 | H5, 5′ | H6 | |
|---|---|---|---|---|---|---|
| Xylose | 5.517 | 3.483 | 3.679 | 3.592 | 3.72 | |
| 3J1,23.5 | 3J2,38.9 | 3J3,48.9 | ||||
| 3J1,P7.0 | 3J2,P3.5 | |||||
| Ribose | 5.938 | 4.34 | 4.255 | 4.34 | 4.15–4.22 | |
| Uracil | 5.956 | 7.927 |
Scalar coupling constants (J, in Hz) for some internal residues are also indicated. Identical results were obtained with standard UDP-Xyl.
