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. 2018 May 30;5(3):ENEURO.0123-18.2018. doi: 10.1523/ENEURO.0123-18.2018

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Copyright © 2018 Bankstahl et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — Concomitant detection of FITC-coupled albumin (FITC-Alb; A) in the pyramidal layer of the hippocampal CA1 region 48 h after SE shows predominantly intracellular FITC-Alb. Neuronal somata are stained with biotinylated anti-NeuN and red fluorescent Cy3-streptavidin (A′), while vascular basement membranes are visualized with rabbit anti-laminin and Cy5-donkey anti-rabbit IgG (A″, immunosignals color-coded in blue). The overlay of staining patterns (A″′) elucidates only rare colocalization of FITC-Alb and NeuN-positive neurons. Scale bar = 50 µm.