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. 2018 May 30;5(3):ENEURO.0123-18.2018. doi: 10.1523/ENEURO.0123-18.2018

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Copyright © 2018 Bankstahl et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — Hippocampal pyramidal cell layer in the CA1 region with FITC-coupled albumin (FITC-Alb; A, B) 48 h following SE and laminin-immunoreactivity in vascular basement membranes (Cy3, red; A′) or endothelial binding sites for biotinylated Solanum tuberosum lectin (STL; Cy3, red; B′), each combined with immunolabeling of astroglial GFAP (A″, B″, Cy5, color-coded in blue) at lower (A–A″′) and higher (B–B″′) magnification. Merged staining patterns (A″′, B″′) elucidate FITC-Alb in close vicinity to laminin-immunopositive structures (arrow in A″′), but no obvious colocalization of FITC-Alb and GFAP-positive astrocytes (A″′, B″′). Scale bars: A″ (also valid for A, A′) = 100 µm, A″′ = 50 µm, B″ (also valid for B, B′) = 50 µm, A″′ = 25 µm.