Figure 6.

Simultaneous demonstration of thalamic FITC-coupled albumin (FITC-Alb) 24 h following SE combined with the detection of the vascular marker collagen IV and binding sites for Solanum tuberosum lectin (STL). FITC-Alb (A) is seen in neuron-like cells in SE-affected regions, mainly marked by apparently up-regulated collagen IV (Coll IV) immunoreactivity (A′, Cy3, red). Concomitant lectin-histochemical staining with STL (A″, Cy5, color-coded in blue) reveals vessels that appear thinner and of lower STL signal in tissue with detectable FITC-Alb. Vascular structures containing both Coll IV– and STL-binding sites appear purple in (A″′). Scale bar = 75 µm.