Sequence alignment of Tdp1 orthologs. The program pileup of
the GCG package was used with some manual modification to create the
alignment. boxshade was used to shade residues black that
are identical in ≥ 50% of the sequences and similar residues
were shaded in gray. The HKD motifs are indicated by black bars above
the alignment. Abbreviations and accession numbers are as follows: hs
(Homo sapiens Tdp1 predicted protein FLJ11090, National
Center for Biotechnology Information RefSeq accession no. NP_060789),
ce (C. elegans, GenPept: AAC68960), dm (D.
melanogaster glaikit protein, GenPept: CAB86488), at (A.
thaliana, GenPept: CAB89327), sp (S. pombe,
hypothetical protein SPCP31B10.05, PIR: T41695), sc (S.
cerevisiae Tdp1, gene product of ORF YBR223c, GenPept:
CAA85186). The C. elegans and A. thaliana
protein sequences were predicted based on genomic sequences.