Fig. 4. The activity of the hemin-Gs in bivalent TSDzyme constructs is dependent on their spacing. (a) Schematic of the bi-hemin-Gs we inserted into TSDzymes. (b) The CD spectra of these confirm that they retain the parallel G-quadruplex conformation of the free G-quadruplex. (c) The catalytic activity of the bi-hemin-Gs increases with increasing spacer length, as shown by the generated Kcat values of various bi-hemin-Gs.
