Table 1.

Sample statistics corresponding to the 18 samples of the study, determined after NextGen sequencing of the V4 variable region of the 16S rRNA gene using the Illumina MiSeq platform. Included are the raw sequences prior to analysis, followed by the quality assessed sequence read counts and the resultant unique observations (unfiltered). This is followed by the filtered count (in which those sequences occurring at <0.0005% were removed), as well as the resultant unique observations. The Shannon and Simpson diversity values corresponding to each sample are also included. For Shannon diversity, a value much higher than 0 would be considered more diverse, and for Simpson diversity, a value closer to 1 would be considered more diverse.

	Raw	Quality	Unique observations	Filtered	Unique observations
Sample	sequences	assessment	(unfiltered)	reads	(filtered)	Shannon	Simpson
Egested fecal pellet 1	204 150	149 503	16 030	133 208	3431	8.33	0.97
Egested fecal pellet 2	158 186	126 394	10 306	117 384	3367	8.14	0.97
Egested fecal pellet 3	138 390	108 855	9538	100 578	3374	8.46	0.98
Gut digesta 1	132 132	105 608	7550	99 468	2554	6.94	0.95
Gut digesta 2	143 149	118 643	5712	114 053	2004	5.76	0.93
Gut digesta 3	113 415	92 589	5131	88 766	2182	7.20	0.96
Gut tissue 1	89 881	74 363	1205	73 264	184	0.83	0.19
Gut tissue 2	125 274	108 088	1627	106 493	173	0.52	0.09
Gut tissue 3	95 653	81 861	1089	80 794	110	0.60	0.12
Pharynx tissue 1	140 831	104 981	3416	101 762	667	4.14	0.67
Pharynx tissue 2	94 593	68 311	2637	65 931	672	4.18	0.76
Pharynx tissue 3	99 972	72 323	1678	71 036	554	2.21	0.38
Seagrass 1	230	175	70	128	41	5.66	0.97
Seagrass 2	696	525	222	470	177	7.23	0.99
Seagrass 3	208 197	137 462	7006	129 410	2053	6.87	0.88
Water 1	494	228	104	195	76	6.01	0.97
Water 2	12 175	9271	1026	8621	568	5.83	0.91
Water 3	3985	2912	439	2692	270	5.73	0.92
Total 18	1761 403	1362 092	74 786	1294 253	22 457