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Species identification by PCR-RFLP. PCR products from the ITS2 region of the nuclear ribosomal RNA gene were obtained from metacercariae of Paragonimus mexicanus (lane 1 and lane 2) and Paragonimus kellicotti (lane 3 and lane 4). The restriction endonuclease Hinc II used for RFLP digested the amplicons from P. mexicanus (lane 2, showing 2 fragments, 380 bp and 140 bp, arrow heads), but not the amplicons from P. kellicotti (lane 4, showing an undigested single fragment of 520 bp).
