Fig 4. Modular Cloning-compatible vectors for a GAL4-based yeast two hybrid system.

(a) Schematic depiction of yeast two hybrid vectors with most important features. (b) Functional verification of vectors shown in (a). Tomato EDS1 and PAD4 were mobilized into vectors shown in (a), resulting constructs co-transformed into yeast cells in the indicated combinations, and co-transformants grown in dilution series on media lacking leucine and tryptophan (-LW), or additionally lacking histidine (-LWH) and adenine (-LWH-Ade). (c) Immunoblot-detection of fusion proteins expressed in yeast cells in (b).