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| Construct targeted to act5 locus | Number of transfectants | Clones checked | Correct clones | Correct clones (%) | Dictyostelium strain used |
|---|---|---|---|---|---|
| LifeAct-mCherry | 7 | 7 | 1 | 14.2 | AX2 |
| LifeAct-GFP | 12 | 12 | 5 | 41.6 | AX2 |
| GFP | 3 | 3 | 2 | 66.6 | AX2 |
| mCherry | 3 | 3 | 1 | 33.3 | AX2 |
| GFP | 66 | 9 | 5 | 55.5 | AX2 |
| mCherry | 221 | 12 | 10 | 83.3 | AX2 |
| H2B-mCherry | 3 | 3 | 1 | 33.3 | AX2 |
| H2B-mCherry | 7 | 7 | 6 | 85.7 | AX2 |
| mCherry | 10 | 10 | 7 | 70 | DdB |
| mCherry | 240 | 12 | 11 | 91.6 | DdB |
| mCherry | 320 | 12 | 12 | 100 | NC4 |
