Table 3. Gene knock-outs generated with cells grown in bacterial suspension.
| Disrupted gene | Number of transfectants | Clones checked | Correct clones | Correct clones (%) | Dictyostelium strain used |
|---|---|---|---|---|---|
| rac1B | 750 | 24 | 23 | 95.8 | NC4 |
| rac1C | 2800 | 24 | 24 | 100 | NC4 |
| rac1A | 500 | 24 | 2 | 8.3 | NC4 |
| axeB (NF1) | 2000 | 15 | 0 | 0 | NC4 |
| scrA (SCAR) | 300 | 11 | 1 | 9.1 | AX2 |
| scrA (SCAR) | 500 | 24 | 1 | 4.1 | AX2 |
| rasS | 22 | 22 | 11 | 50 | AX2 |
| rasS | 12 | 12 | 3 | 25 | DdB |
| corA | 12 | 12 | 11 | 91.6 | AX2 |
| myoC | 12 | 12 | 10 | 83.3 | AX2 |
| erkB | 28 | 28 | 3 | 10.7 | DdB |
| erkB | 6 | 6 | 1 | 16.6 | DdB |
| erkB | 4 | 4 | 1 | 25 | DdB |
| erkB | 5 | 5 | 0 | 0 | DdB |
| racA | 24 | 24 | 10 | 41.6 | AX2 |
| rasS | 24 | 24 | 10 | 41.6 | AX2 rasG- |
| rasC | 96 | 96 | 2 | 2 | AX2 |
| pkbR1 | 36 | 36 | 9 | 25 | AX2 |
| pkbR1 | 29 | 29 | 3 | 10.3 | AX2 akt- |
| myoC | 203 | 12 | 10 | 83.3 | AX2 myoB/E- |
| myoC | 181 | 12 | 12 | 100 | AX2 myoE/F- |
| forG | 58 | 58 | 2 | 3.4 | AX2 |