Table 4. Removal of the selectable marker with cells grown in bacterial suspension.
Gene | Number of transfectants | Clones checked | Correct clones | Correct clones (%) | Dictyostelium strain used |
---|---|---|---|---|---|
myoB | 198 | 8 | 6 | 75 | AX2 myoB/E- |
myoF | 197 | 13 | 13 | 100 | AX2 |
act5::H2B-mCherry | 74 | 7 | 7 | 100 | AX2 |