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. 2018 May 30;13(5):e0197349. doi: 10.1371/journal.pone.0197349

Fig 4. Off-tumor effect of inducible CD38-CAR T cells.

Fig 4

(A) Representative flow cytometry density plots of MM-BM with CD38+/CD138+ cells (MM) after treatment with inducible mock (+/- 1000 ng/ml DOX for 48 hours) and inducible high affinity (028) CD38-CAR T cells (- DOX or + 1000 ng/ml DOX for 48 hours and 0, 24 or 120 hours after DOX removal). (B) Pooled data obtained from the analysis of five MM patient bone marrow samples (patient 1–5, see for their phenotype data S4 Fig) with ~20% MM cells (S4 Fig) were co-incubated with inducible high affinity (028) CD38-CAR T cells (E:T ratio 3:1) treated with DOX according to the schedule Fig 3A. Shown are the mean CAR-dependent % lysis of MM (CD138+/CD38+ ; open squares) and % lysis of healthy non-MM cells (CD138-/CD56-/CD38+/-; grey diamonds) by inducible CD38-CAR T cells. Incubated with DOX for 24 hours 1000 ng/ml (upper left), 48 hours 1000 ng/ml (upper right), 24 hours 10 ng/ml (lower left), 48 hours 10 ng/ml (lower right). Presented is the pooled data from 5 independent experiments. Error bars indicate the mean +/- SEM. (Pt 1–5, S4 Fig).