Fig 1. Albumin-cre, Ildr2 KO mice do not develop hepatic steatosis.

(A) Schematic of the floxed Ildr2 allele (not to scale). (B,C) Expression of Ildr2, isoforms 1 and 4 in livers of 23-week old Ildr2^Alb KO mice and littermate Ildr2^fl/fl controls, fed chow or HFD for 17 weeks. Expression was measured by qPCR and normalized to 36b4, actb and Gapdh expression. (D) Body weight curves of HFD and chow-fed, Ildr2^Alb KO mice. (E,F) Percent fat mass and lean mass of HFD and chow-fed, Ildr2^Alb KO mice measured weekly by NMR. (G) Photographs of livers excised from HFD and chow-fed, Ildr2^Alb KO mice at 23 weeks of age. (H) Hematoxylin and eosin staining of representative liver sections at 50X magnification. (I) Liver weight at 23 weeks of age. (J,K) Liver triglyceride and total cholesterol content (measured in duplicate). (L,M) Plasma triglyceride and total cholesterol concentration at 23 weeks of age after a 4hr. fast. n = 4–5 mice per group. Data are represented as mean ± standard error (SEM) * p<0.05, ** p<0.01, *** p<0.001 for Ildr2^Alb KO vs. Ildr2^fl/fl control. + p<0.05, ++ p<0.01, +++ p<0.001 for chow vs. HFD.