Figure 2.

Transfection of HMC cells with Tag and SV40 DNA. (A) Tag sequence (*) was amplified by PCR from genomic DNA of COS-7 (control), HMC, Tag-HMC, and SV40-HMC cells. (B) Tag protein (*) was detected in cell lysates immunoprecipitated and probed with Tag antibodies. (C) Immunoblotting was performed on Met immunoprecipitates with Met antibodies. GTL-16 cell line was used as a control of Met expression and phosphorylation. Asterisks on the right of the blot indicate the positions of the pr170^MET precursor (**) and p145^MET (*). (D) The same filter was reprobed with antiphosphotyrosine antibodies. (E) Citofluorimeter analysis of HMC cells before (Left) and after (Right) transfection with SV40 DNA.