Figure 3.

Morphology after cocultures with SV40-HMC. The morphology of target cells CV-1 (A), BNL CL (D), and HMC (G) changed after coculture with SV40-HMC cells (B, E, and H, respectively). SV40 particles were observed by electron microscopy (C and I). (F) No virions were detected in nonpermissive BNL CL cells. CV-1 cells underwent vacuolization and massive lysis, accompanied by Tag expression. Tag protein (*) was detected in cell lysates immunoprecipitated and probed with Tag antibodies. (Original magnifications: A, B, D, E, G, and H, ×320; C, ×50,000; F and I, ×40,000.)